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KMID : 0380020150300030132
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Korean Journal of Biotechnology and Bioengineering
2015 Volume.30 No. 3 p.132 ~ p.139
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Stabilization and Antifungal Activity of Isolated Symbiotic Bacteria from Entomopathogenic Nematodes
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Kang Dong-Hee
Kim Hyo-Hyun
Nam Uk-Ho
Kim Hyun-Soo
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Abstract
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In order to use the symbiotic bacteria from ethomophatogenic nematodes as a biological control agent for agriculture, the cultural condition for maintaining phase I and antifungal activity was investigated. Symbiotic bacteria (SB) 1 stain from nematodes were selected from the three strains isolated from entomopathogenic nematodes. The growth of the SB 1 strain in NB, TSB, TY and YS medium was higher than that of the SB 2 and SB 3 strain. The packed cell volume of the SB 1 strain was reduced in NB medium which showed radical pH change. Phase I of the SB 1 strain was maintained in TSB medium after being stored for 2 weeks at 4°C. Culture broth with the SB 1 strain in TSB medium for 6 days and 7 days showed antifungal activities against Rhizoctonia solani KACC 40142, Botrytis cinerea Pers. KACC 40854, and Botrytis cinerea Pers. KACC 41008. Culture broth with the SB 1 strain in TSB medium containing 100 mM L-proline for 5 days showed antifungal activities against Rhizoctonia solani KACC 40142, and Botrytis cinerea Pers. KACC 40854.
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KEYWORD
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Symbiotic bacteria, Entomopathogenic nematodes, Antifungal activity, Phase change, Stabilization
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